產(chǎn)品信息
名稱(chēng) HS-27A (人骨髓細(xì)胞) (STR鑒定正確)
別稱(chēng) HS27A; HS27a
種屬 人類(lèi)
生長(zhǎng)特性 貼壁細(xì)胞
細(xì)胞形態(tài) 成纖維細(xì)胞樣
凍存條件 凍存液:55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO溫度:液氮
培養(yǎng)方案A(默認(rèn)) 生長(zhǎng)培養(yǎng)基:RPMI-1640+10%FBS+1%P/S培養(yǎng)條件:氣相:空氣,95%;CO2,5%, 溫度:37℃
推薦傳代比例 1:2-1:3
推薦換液頻率 2-3次/周
參考資料(來(lái)源文獻(xiàn))
背景描述 HS-27A is a fibroblast cell line that was isolated in (year from History) from the stroma of a 30-year-old White male donor. HS-27A is a subclone of HS-27.HS-27A forms large flattened polygonal shaped cells that exemplify "blanket" cells and maintain numerous intercellular contacts with neighboring cells. Unlike HS-5, HS-27A secretes low levels of growth factors and does not support proliferation of isolated progenitor cells in cocultures. HS-27A expresses relatively high levels of vascular cell adhesion molecule 1 (VCAM-1).
年齡(性別) 男性;30歲
組織來(lái)源 骨頭;骨髓;基質(zhì)
細(xì)胞類(lèi)型 轉(zhuǎn)化細(xì)胞系
生物安全等級(jí) BSL-2
倍增時(shí)間 ~36h-48 hours
抗原表達(dá)情況 Vascular cell adhesion molecule 1 (CD106, VCAM-1)
應(yīng)用領(lǐng)域 3D cell culture; Immunology
保藏機(jī)構(gòu) ATCC; CRL-2496