ATCC? Number:PCS-200-012
Name: Primary Epidermal Melanoyctes; Normal, Human, Neonatal
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Foreskin, lightly pigmented
Growth Properties: Adherent
Age: Neonatal
Morphology: Stellar, multipolar or dendritic apperance; needle-like
Number of Viable Cells per Vial: ≥ 500,000 in cryopreservation medium
Volume per Vial: 1 ml
Gender: Male
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Related Products:Dermal Cell Basal Medium (ATCCPCS-200-030)
Melanocyte Growth Kit (ATCCPCS-200-041)
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC30-2200)
Trypsin-EDTA for Primary Cells (ATCCPCS-999-003)
Trypsin Neutralizing Solution (ATCCPCS-999-004)
Gentamicin-Amphotericin B Solution (ATCCPCS-999-025)
Penicillin-Streptomycin-Amphotericin B Solution (ATCCPCS-999-002)
Phenol Red (ATCCPCS-999-001)
Comments:ATCC? Normal Human Primary Epidermal Melanocytes from Neonatal Foreskin, when grown in Dermal Cell Basal Media supplemented with Melanocyte Growth Kit components, provide an ideal cell system to propagate melanocytes in low serum (less than 1.0% FBS) conditions in the absence of cholera toxin and phorbol 12-myristate 13-acetate (PMA). The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency.
Applications:Melanoma, response to UV radiation, psoriasis and other skin diseases, skin trauma (e.g., wound repair, scars, burns), cosmetic research (e.g., skin lightening compounds, skin protecting compounds)
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests:Bacteria and Yeast: Negative Mycoplasma: Negative Hepatitis B Testing: Negative
Hepatitis C Testing: Negative
HIV-1 Testing: Negative