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ATCC? Number:PCS-200-011
Name: Primary Epidermal Keratinocytes; Normal, Human, Adult
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Skin (epidermis)
Growth Properties: Adherent
Age: Adult
Morphology: Cobblestone appearance; cells are rounded, not flat
Number of Viable Cells per Vial: ≥ 500,000 in cryopreservation medium
Volume per Vial: 1 ml
Ethnicity: Lot-specific
Gender: Lot-specific
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Primary Epidermal Keratinocytes; Normal, Human, Neonatal Foreskin (ATCCPCS-200-010)
Dermal Cell Basal Medium (ATCCPCS-200-030)
Keratinocyte Growth Kit (ATCCPCS-200-040)
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC30-2200)
Trypsin-EDTA for Primary Cells (ATCCPCS-999-003)
Trypsin Neutralizing Solution (ATCCPCS-999-004)
Gentamicin-Amphotericin B Solution (ATCCPCS-999-025)
Penicillin-Streptomycin-Amphotericin B Solution (ATCCPCS-999-002)
Phenol Red (ATCCPCS-999-001)

Comments:A complete solution to propagate keratinocytes isolated from human skin. The serum-free media is formulated to inhibit fibroblast growth, and the low calcium concentration (60 μM) slows differentiation. No feeder layers, extracellular matrix proteins or other substrates are required.
Applications:Toxicology, wound repair, skin cancer, response to UV radiation, psoriasis, eczema, viral infection, gene delivery systems, cellular differentiation, cosmetics research/testing
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium