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ATCC? Number:PCS-100-011
Name: Primary Aortic Endothelial Cells; Normal, Human
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Aortic endothelium
Growth Properties: Adherent
Age: Adult
Morphology: Cobblestone appearance
Number of Viable Cells per Vial: ≥ 500,000 in cryopreservation medium
Volume per Vial: 1 ml
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Vascular Cell Basal Medium (ATCCPCS-100-030)Endothelial Cell Growth Kit-BBE (ATCCPCS-100-040)Endothelial Cell Growth Kit-VEGF (ATCCPCS-100-041)Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC30-2200)Trypsin-EDTA for Primary Cells (ATCCPCS-999-003)Trypsin Neutralizing Solution (ATCCPCS-999-004)Gentamicin-Amphotericin B Solution (ATCCPCS-999-025)Penicillin-Streptomycin-Amphotericin B Solution (ATCCPCS-999-002)Phenol Red (ATCCPCS-999-001)
Comments:A complete solution to propagate Aortic Endothelial cells in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more authentic, less defined culture medium is desired.
Applications:Arteriolosclerosis, hypertension, arterial disease, vascular wound healing, inflammation, tissue remodeling, vascular differentiation, apoptosis, drug screening, stent development.
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests:Bacteria and Yeast: Negative
Mycoplasma: Negative

Testing:Hepatitis B: Negative Hepatitis C: Negative HIV-1: Negative