名稱 THLE-2 (人肝永生化細(xì)胞) (STR鑒定正確)
別稱 THLE2; Transformed Human Liver Epithelial-2
種屬 人
生長特性 貼壁細(xì)胞
細(xì)胞形態(tài) 上皮細(xì)胞樣
凍存條件 凍存液：55%DM/F12+40%FBS+5%DMSO溫度：液氮
培養(yǎng)方案A（默認(rèn)） 生長培養(yǎng)基:THLE-2 專用培養(yǎng)基培養(yǎng)條件:氣相：空氣，95%；CO2，5%, 溫度：37℃
推薦傳代比例 1:3-1:6
推薦換液頻率 2-3次/周
參考資料(來源文獻(xiàn))
背景描述 THLE-2 is an epithelial cell that was isolated from the left lobe of a donor.THLE-2 and THLE-3 cells express phenotypic characteristics of normal adult liver epithelial cells. They are nontumorigenic when injected into athymic nude mice, have near-diploid karyotypes, and do not express alpha-fetoprotein. [RF84750] THLE-2 and THLE-3 cells metabolize benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 to their ultimate carcinogenic metabolites that adduct DNA, which indicates functional cytochrome P450 pathways.
年齡（性別） 男性
組織來源 肝臟；左葉
細(xì)胞類型 轉(zhuǎn)化細(xì)胞系
生物安全等級 BSL-2
致瘤性 No; No, nude mice
保藏機(jī)構(gòu) ATCC; CRL-2706
