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描述：小鼠全能性胚胎干細胞，每支細胞量1×106

 

動物種別：小鼠，129S4/SvJae品系

 

性別：雄

 

組織來源：小鼠胚胎內細胞團

 

形態(tài)：球形克隆

 

培養(yǎng)基和添加劑：mES完全培養(yǎng)液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES級FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（終濃度為1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

 

供應限制：A。僅供研究之用。

 

REFERENCE：Stevens LC. A new inbred subline of mice (129-terSv) with a high incidence of spontaneous congenital testicular teratomas. J. Natl. Cancer Inst. 50: 235-242, 1973. PubMed: 4692863 Li E, et al. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69: 915-926, 1992. PubMed: 1606615 Krzyzanowski PM, et al. Identification of novel stem cell markers using gap analysis of gene expression data. Genome Biology 8(9): R193, 2007. PubMed: 17875203 Luikenhuis S, et al. Antisense transcription through the Xist locus mediates Tsix function in embryonic stem cells. Mol. Cell Biol. 21(24): 8512?8520, 2001. PubMed: 11713286 Goto T, et al. Regulation of X-Chromosome inactivation in development in mice and humans. Micro. Molec. Biol. Rev. (ASM) 62(2): 362-378, 1998. PubMed: 9618446 Yap DYL, et al. Using biomarker signature patterns for an mRNA molecular diagnostic of mouse embryonic stem cell differentiation state. BMC Genomics 8: 210, 2007. PubMed: 17605829 Haase VH, et al. Vascular tumors in livers with targeted inactivation of the von Hippel?Lindau tumor suppressor. Proc. Natl. Acad. Sci. 98(4): 1583?1588, 2001. PubMed: 11171994 Oda M, et al. DNA methylation regulates long-range gene silencing of an X-linked homeobox gene cluster in a lineage-specific manner. Genes Dev. 20(24): 3382-3394, 2006. PubMed: 17182866 Lorincz MC, et al. DNA methylation density influences the stability of an epigenetic imprint and Dnmt3a/b-independent de novo methylation. Mol. Cell Biol. 22(21): 7572?7580, 2002.PubMed: 12370304 Tucker KL, et al. A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res. 25: 3745-3746, 1997. PubMed: 9278500 Biniszkiewicz D, et al. Dnmt1 overexpression causes genomic hypermethylation, loss of imprinting, and embryonic lethality. Mol. Cell Biol. 22(7): 2124-2135, 2002. PubMed: 11884600

 

中文名稱：小鼠胚胎干細胞J1

提供形式：復蘇狀態(tài)