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平臺(tái)編號(hào)：bio-54248
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細(xì)胞信息： C57BL/6
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：種屬鑒定正確
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
小鼠胚胎干細(xì)胞
細(xì)胞名稱：C57BL/6小鼠胚胎干細(xì)胞C57BL/6
描述；小鼠全能性胚胎干細(xì)胞，每支細(xì)胞量1×106
動(dòng)物種別；小鼠，C57BL/6品系
性別：
組織來源：小鼠胚胎內(nèi)細(xì)胞團(tuán)
形態(tài)：球形克隆
培養(yǎng)基和添加劑：mES完全培養(yǎng)液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES級(jí)FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（終濃度為1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。
供應(yīng)限制:A。僅供研究之用。
REFERENCE；Brook FA, et al. The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome. Diabetes. 52:205-208, 2003. PubMed: 12502514 Brook FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl. Acad. Sci. USA. 94: 5709-5712, 1997. PubMed: 9159137 Schuster-Gossler K, et al. Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines. BioTechniques 31: 1022-1024, 1026, 2001. PubMed: 11730008 Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865 Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach.Oxford, Oxford University Press, 101-132, 1999 Lemckert FA, et al. Gene targeting in C57BL/6 ES cells. Successful germ line transmission using recipient BALB/c blastocysts developmentally matured in vitro. Nucleic Acids Res. 25: 917-918, 1997. PubMed: 9016649 Ledermann B, Burki K. Establishment of a germ-line competent C57BL/6 embryonic stem cell line. Exp. Cell Res. 197: 254-258, 1991. PubMed: 1959560
中文名稱:小鼠胚胎干細(xì)胞C57BL/6
提供形式：復(fù)蘇狀態(tài)
