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平臺(tái)編號(hào)：bio-54284
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細(xì)胞信息： AB2.2
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：種屬鑒定正確
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
小鼠胚胎干細(xì)胞AB2.2
細(xì)胞名稱(chēng)：AB2.2小鼠胚胎干細(xì)胞AB2.2
描述；小鼠全能性胚胎干細(xì)胞，每支細(xì)胞量1×106
動(dòng)物種別；小鼠，129S5/SvEvBrd（原129/SvEvBrd）品系
性別：雄
組織來(lái)源：小鼠胚胎內(nèi)細(xì)胞團(tuán)
形態(tài)：球形克隆
培養(yǎng)基和添加劑：mES完全培養(yǎng)液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES級(jí)FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（終濃度為1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。
供應(yīng)限制 A。僅供研究之用。
REFERENCE；Bader A, Al-Dubai H, Weitzer G. Leukemia Inhibitory Factor Modulates Cardiogenesis in Embryoid Bodies in Opposite Fashions. Circ. Res. 86: 787-794, 2000. PubMed: 10764413 Wolcik SM, Longley MA, Roop DR. Discovery of a novel murine keratin 6 (K6) isoform explains the absence of hair and nail defects in mice deficient for K6a and K6b. J. Cell Biol. 154: 619-630, 2001. PubMed: 11489919 Liu Y, et al. Sox17 is essential for the specification of cardiac mesoderm in embryonic stem cells. Proc Natl Acad Sci USA. 104(10): 3859-3864, 2007. PubMed: 17360443 Adams DJ, et al. A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction. Genomics 86(6): 753-758. 2005. PubMed: 16257172 Festing MF, et al. Revised nomenclature for strain 129 mice. Mamm. Genome. 10(8):836, 1999.PubMed: 10430671 Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391 Bradley A, Zheng B, Liu P. Thirteen years of manipulating the mouse genome: a personal history. Int. J. Dev. Biol. 42 (7): 943-950, 1998. PubMed: 9853825
中文名稱(chēng):小鼠胚胎干細(xì)胞AB2.2
提供形式：復(fù)蘇狀態(tài)
