![DUKX B1 [5A HS MYC]](http://www.family998.com/static/images/cells-no_picture.jpg)
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平臺(tái)編號(hào)：bio-68055
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國際編號(hào)：CRL-9010?
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細(xì)胞信息： DUKX B1 [5A HS MYC]
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
DUKX B1 [5A HS MYC]
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-9010?
器官來源：卵巢
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：倉鼠
生長狀態(tài)：貼壁生長
細(xì)胞形態(tài)：上皮樣
規(guī)格：0.5mg Designations: DUKX B1 [5A HS MYC]
Depositors: ?R Kingston
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent, adherent
Organism: Cricetulus griseus
Morphology:epithelial
Source: Organ: ovary
Cellular Products:over produces mouse c-myc protein when heated
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: female
Comments:The cells are reported to contain DNA sequences from mouse, drosophila, adenovirus and E. coli.
Propagation: ATCC complete growth medium: Alpha modified Eagle's minimum essential medium with 0.32 mM methotrexate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium ,95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
ATCC Number：CRL-9010?
器官來源：卵巢
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：倉鼠
生長狀態(tài)：貼壁生長
細(xì)胞形態(tài)：上皮樣
規(guī)格：0.5mg Designations: DUKX B1 [5A HS MYC]
Depositors: ?R Kingston
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent, adherent
Organism: Cricetulus griseus
Morphology:epithelial
Source: Organ: ovary
Cellular Products:over produces mouse c-myc protein when heated
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: female
Comments:The cells are reported to contain DNA sequences from mouse, drosophila, adenovirus and E. coli.
Propagation: ATCC complete growth medium: Alpha modified Eagle's minimum essential medium with 0.32 mM methotrexate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium ,95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
