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細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：大鼠
運輸方式：凍存運輸
數(shù)量：大量
生長狀態(tài)：混合型生長
ATCC Number：CRL-1569?
器官來源：腎臟
相關(guān)疾?。赫?br /> 規(guī)格：0.2ml Designations: KNRK
Depositors: ?JE DeLarco
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:mixed: adherent and suspension
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:

Source: Organ: kidney
Disease: normal
Cell Type: Kirsten murine sarcoma virus (Ki-MSV) transformed
Cellular Products:The cells release growth factors in serum free medium.
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:The cells do not produce Ki-MSV particles.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by transferring floating cells to additional flasks.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 22501: Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712
32931: Gimona M, et al. Forced expression of tropomyosin 2 or 3 in v-Ki-ras-transformed fibroblasts results in distinct phenotypic effects. Proc. Natl. Acad. Sci. USA 93: 9618-9623, 1996. PubMed: 8790379
33135: Bohm SK, et al. Mechamisms of desensitization and resensitization of proteinase-activated receptor-2. J. Biol. Chem. 271: 22003-22016, 1996. PubMed: 8703006