生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來源：乳房
數(shù)量：大量
細(xì)胞形態(tài)：上皮樣
ATCC Number：CRL-2865?
相關(guān)疾?。簩?dǎo)管癌
年限：54 years adult
組織來源：duct
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：人
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5mg Designations: T47D-KBluc
Depositors: ?VS Wilson
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: mammary gland; breast
Tissue: duct
Disease: ductal carcinoma
Derived from metastatic site: pleural effusion
Cell Type: epithelial transfected with reporter plasmid
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: January 4, 2000
Applications:They can be used to screen chemicals for estrogenic or anti-estrogenic activity.
Reverse Transcript:N
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14
Age: 54 years adult
Gender: female
HeLa Markers: N
Comments:The parent cell line was transfected with pGL2.TATA.Inr.luc.neo which contains three estrogen responsive elements upstream of a luc reporter gene. The cells were selected for responsiveness to 17beta-estradiol. They can be used to screen chemicals for estrogenic or anti-estrogenic activity.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.2 Units/ml bovine insulin; fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Duration: The depositor states that supplementing the growth medium with insulin is optional.
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with either Hanks' Balanced Salt Solution (HBSS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. - Add 10 to 15 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(4) to 4 X 10(4) viable cells/sq. cm is recommended.
- Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentration between 3 X 10(4) and 2 X 10(5) cells/sq. cm.
Subcultivation Ratio: 1:2 to 1:3 is recommended
Medium Renewal: every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 36
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC HTB-133
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Hanks' Balanced Salt Solution (w/o Ca++, Mg++, phenol red):ATCC 30-2213
References: 89919: Wilson VS, et al. Development and characterization of a cell line that stably expresses an estrogen-responsive luciferase reporter for the detection of estrogen receptor agonist and antagonists. Toxicol. Sci. 81: 69-77, 2004. PubMed: 15166400
