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器官來源：骨髓
細胞形態(tài)：淋巴樣
ATCC Number：CRL-2420?
數(shù)量：大量
年限：adult
生長狀態(tài)：懸浮生長，少量貼壁
品系：C3H
是否是腫瘤細胞：0
物種來源：小鼠
運輸方式：凍存運輸
規(guī)格：0.5mg Designations: LADMAC
Depositors: ?WS Walker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, with some loosely adherent cells
Organism: Mus musculus deposited as mouse
Morphology:lymphoblast


Source: Organ: bone marrow
Strain: C3H
Cellular Products:colony stimulating factor-1 (CSF-1)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Age: adult
Comments:LADMAC is a transformed cell line derived by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with cloned human cellular myc-homologous sequences covalently attached to pBR325 (pR myc).
The cell line has monocyte-like morphology; contains nonspecific esterase; is phagocytic for latex beads; secretes lysozyme, and bears the Mac-1 antigen.
A minority of cells are Fc receptor positive and an appreciable number of cells are complement receptor 1 positive.
The cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. The cells are not phagocytic for antibody or complement-coated particles; they do not constitutively secrete Interleukin-1.
LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. [38883 ]
The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1. [38884 ]
This cell line is used to produce LADMAC conditioned medium. It will support the growth of the macrophage cell lines EOC 2 (CRL-2467), EOC 13.31 (CRL-2468), EOC 20 (CRL-2469), I-11.15 (CRL-2470) and I-13.35 (CRL-2471).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 38883: Sklar MD, et al. Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730
38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247