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平臺編號：bio-68083
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國際編號：CRL-2227?
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細(xì)胞信息： AP.6
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
AP.6
生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
ATCC Number：CRL-2227?
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
數(shù)量：大量
規(guī)格：0.5mg Designations: AP.6
Depositors: ?FM Brodsky
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against AP-2 adaptor protein of clathrin coated vesicles
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Note: These cells are distributed for research purposes only subject to the following: 1) The AP.6 cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 2) Any proposed commercial use of these cells or their products must first be negotiated with Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 3) In all papers reporting any use of these cells or derived products, a direct reference will be made to Proc. Natl. Acad. Sci. USA 86:9289-9293, 1989.
Comments:Animals were immunized with adaptor protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the alpha chain of the AP-2 protein of all mammalian species tested.
It is useful for immunofluorescence and immunoprecipitation assays, but tends to give spurious bands in Western blots.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23316: Chin DJ, et al. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc. Natl. Acad. Sci. USA 86: 9289-9293, 1989. PubMed: 2574457
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
ATCC Number：CRL-2227?
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
數(shù)量：大量
規(guī)格：0.5mg Designations: AP.6
Depositors: ?FM Brodsky
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against AP-2 adaptor protein of clathrin coated vesicles
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Note: These cells are distributed for research purposes only subject to the following: 1) The AP.6 cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 2) Any proposed commercial use of these cells or their products must first be negotiated with Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 3) In all papers reporting any use of these cells or derived products, a direct reference will be made to Proc. Natl. Acad. Sci. USA 86:9289-9293, 1989.
Comments:Animals were immunized with adaptor protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the alpha chain of the AP-2 protein of all mammalian species tested.
It is useful for immunofluorescence and immunoprecipitation assays, but tends to give spurious bands in Western blots.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23316: Chin DJ, et al. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc. Natl. Acad. Sci. USA 86: 9289-9293, 1989. PubMed: 2574457
