ATCC Number：TIB-180?
相關疾?。喊籽?br />
細胞類型：其他細胞類型
運輸方式：凍存運輸
數(shù)量：大量
是否是腫瘤細胞：0
物種來源：人
生長狀態(tài)：懸浮生長
年限：30 years
細胞形態(tài)：淋巴樣
器官來源：骨髓
規(guī)格：48T Designations: HEL 92.1.7
Depositors: ?PJ Martin
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: bone marrow
Disease: erythroleukemia
Cell Type: erythroblast
Cellular Products:hemoglobin; globin (G gamma, A gamma, epsilon, eta and alpha chains); beta-2-microglobulin; glycophorin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:These cells differentiate spontaneously into erythroblast-like cells.
Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid).
Antigen Expression:HLA A3, Aw32, Bw35; Ia+
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 7
THO1: 7
TPOX: 11
vWA: 14,17
Age: 30 years
Gender: male
Ethnicity: Caucasian
Comments:These cells differentiate spontaneously into erythroblast-like cells.
Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22614: Papayannopoulou T, et al. Human erythroleukemia cell line (HEL) undergoes a drastic macrophage- like shift with TPA. Blood 62: 832-845, 1983. PubMed: 6309287
23500: Martin P, Papayannopoulou T. HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science 216: 1233-1235, 1982. PubMed: 6177045
32395: Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679
32446: Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663
33012: Riewald M, et al. Identification and characterization of the cytoplasmic antiproteinase (CAP) in human platelets. J. Biol. Chem. 271: 7160-7167, 1996. PubMed: 8636153
