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平臺(tái)編號(hào)：bio-68095
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國(guó)際編號(hào)：CRL-10318?
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細(xì)胞信息： MCF 10F
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
MCF 10F
年限：36 years
ATCC Number：CRL-10318?
相關(guān)疾?。浩渌膊?br /> 是否是腫瘤細(xì)胞：0
物種來源：人
運(yùn)輸方式：凍存運(yùn)輸
器官來源：乳房
細(xì)胞形態(tài)：上皮樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
規(guī)格：0.1ml Designations: MCF 10F
Depositors: ?Michigan Cancer Foundation
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: mammary gland; breast
Disease: fibrocystic disease
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:epidermal growth factor (EGF), expressed
insulin, expressed
glucocorticoid, expressed
Tumorigenic:No
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 15,17
Cytogenetic Analysis:aneuploid human female; 46, XX, 1p+, t(3:9)(p13:p22)
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1-2
PGM3, 1
Age: 36 years
Gender: female
Comments:The MCF 10F cell line is a non-tumorigenic epithelial cell line.
The line was produced by long term culture in serum free medium with low Ca++ concentration.
MCF 10F was derived from floating cells in the population.
Cells derived from an adherent population are available (see MCF 10A, ATCC CRL-10317 ).
The cells are positive for epithelial sialomucins.
Cytokeratins and milk fat globule antigen.
They exhibit three dimensional growth in collagen, and form domes in confluent cultures.
Thus far, the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF). [21968 ]
By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells.
They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.
The calcium content of the medium exerts a strong effect on the morphology of the cells.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with reduced Ca++ (0.04 mM final), 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.01 mg/ml insulin and 500 ng/ml hydrocortisone, 95%; Chelex-treated horse serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:derived from same individual:ATCC CRL-10317
derived from same individual:ATCC CRL-10780
derived from same individual:ATCC CRL-10781
References: 21968: Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991
22025: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993
22248: Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007
23084: Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513
23085: Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506
ATCC Number：CRL-10318?
相關(guān)疾?。浩渌膊?br /> 是否是腫瘤細(xì)胞：0
物種來源：人
運(yùn)輸方式：凍存運(yùn)輸
器官來源：乳房
細(xì)胞形態(tài)：上皮樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
規(guī)格：0.1ml Designations: MCF 10F
Depositors: ?Michigan Cancer Foundation
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: mammary gland; breast
Disease: fibrocystic disease
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:epidermal growth factor (EGF), expressed
insulin, expressed
glucocorticoid, expressed
Tumorigenic:No
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 15,17
Cytogenetic Analysis:aneuploid human female; 46, XX, 1p+, t(3:9)(p13:p22)
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1-2
PGM3, 1
Age: 36 years
Gender: female
Comments:The MCF 10F cell line is a non-tumorigenic epithelial cell line.
The line was produced by long term culture in serum free medium with low Ca++ concentration.
MCF 10F was derived from floating cells in the population.
Cells derived from an adherent population are available (see MCF 10A, ATCC CRL-10317 ).
The cells are positive for epithelial sialomucins.
Cytokeratins and milk fat globule antigen.
They exhibit three dimensional growth in collagen, and form domes in confluent cultures.
Thus far, the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF). [21968 ]
By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells.
They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.
The calcium content of the medium exerts a strong effect on the morphology of the cells.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with reduced Ca++ (0.04 mM final), 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.01 mg/ml insulin and 500 ng/ml hydrocortisone, 95%; Chelex-treated horse serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:derived from same individual:ATCC CRL-10317
derived from same individual:ATCC CRL-10780
derived from same individual:ATCC CRL-10781
References: 21968: Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991
22025: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993
22248: Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007
23084: Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513
23085: Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506
