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器官來源：皮膚
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
細(xì)胞類型：上皮細(xì)胞
是否是腫瘤細(xì)胞：0
生長狀態(tài)：貼壁生長
ATCC Number：CRL-2872?
細(xì)胞形態(tài)：上皮樣
規(guī)格：1mg Designations: EPC [Epithelioma Papulosum Cyprini]
Depositors: ?J Winton
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Morphology:epithelial


Source: Organ: skin
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1969
Virus Susceptibility:Infectious pancreatic necrosis virus
Infectious hematopoietic necrosis virus
Spring Viremia of Carp Virus
Viral hemorrhagic septicemia virus
Epizootic hematopoietic necrosis virus
Largemouth bass virus
Comments:The EPC line has a broad sensitivity for fish viruses. It is used internationally for isolation, propagation and diagnostic assays for fish viruses.. CRL-2872, originally deposited as Carp (Cyprinus carpio ), was subsequently found to be Fathead Minnow (Pimephales promelas ) through cytochrome c oxidase subunit I testing at ATCC .
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 25.0°C
Max Temperature: 30.0°C
Min Temperature: 20.0°C
Subculturing: Protocol: Volumes used in this protocol are for 75 sq. cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes).
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 X 10(3) to 7 X 10(3) viable cells/sq. cm is recommended.
6. Incubate cultures at 25C. We recommend that you maintain cultures at a cell concentration between 1 X 10(5) and 2 X 10(5) cells/sq. cm.

Subcultivation Ratio: 1:4 to 1:6
Preservation: Freeze medium: culture medium, 95%; DMSO,5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: about 36 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Cell culture tested DMSO:ATCC 4-X
Erythrosin B vital stain solution:ATCC 30-2404
Trypan Blue vital stain solution:ATCC 30-2402
References: 92448: Fijan N, et al. Some properties of the Epithelioma Papulosum Cyprini (EPC) cell line from carp Cyprinus carpio. Ann. Virol. (Inst. Pasteur) 134 E: 207-220, 1983.
92449: Fryer JL, Lannan CN. Three decades of fish cell culture: A current listing of cell lines derived from fishes. J. Tissue Culture Methods 16: 87-94, 1994.
92450: Lorenzen E, et al. Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV. Dis Aquat Org 37: 81-88, 1999. PubMed: 10494498
92451: Loh PC, et al. Growth of the penaeid shrimp virus infectious hypodermal and hematopoietic necrosis virus in a fish cell line. J. Virol. Methods 28: 273-280, 1990. PubMed: 2117021