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組織來(lái)源：cerebral cortex
相關(guān)疾病：其他疾病
ATCC Number：CRL-2299?
品系：BALB/c
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
物種來(lái)源：小鼠
是否是腫瘤細(xì)胞：0
細(xì)胞形態(tài)：內(nèi)皮樣
細(xì)胞類型：其他細(xì)胞類型
運(yùn)輸方式：凍存運(yùn)輸
年限：6 weeks
器官來(lái)源：大腦
數(shù)量：大量
規(guī)格：0.1ml Designations: bEnd.3
Depositors: ?EC Butcher
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:endothelial


Source: Organ: brain
Strain: BALB/c
Tissue: cerebral cortex
Disease: endothelioma
Cell Type: endothelialpolyoma middle T antigen transformed
Cellular Products:von Willebrand factor
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The bEnd.3 cell line is provided for research use only under the following terms:1. bEnd.3 cells, or products derived from them, must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, bEnd.3 cells or their products. Commercial interests are the exclusive property of The Max Planck Society. 2. Any proposed commercial use of bEnd.3 cells, or products derived from them, must first be negotiated with Dr. Britta Engelhardt, on behalf of the late Dr. Werner Risau, Max-Planck-Institut fur physiologische und klinische Forschung, W.G. Kerckoff Institut, Abteilung molekulare Zellbiologie, Parkstrasse 1, 61231 Bad Nauheim, Germany. Telephone: 49-6032-705203/265; FAX: 49-6032-072259; email: b.engelhardt@kerckoff.mpg.de3. In all papers reporting any use of bEnd.3 cells, or products derived from them, a direct reference will be made to the original publication (Montesano, et al. Cell 62:435-445, 1990).
Antigen Expression:ICAM-1 +; VCAM-1 +; MAdCAM-1 +
Age: 6 weeks
Comments:The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen. [39039 ]
The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL). [39039 ]
The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd.3 cells by cytokines and lipopolysaccharide (LPS). [39040 ]
This induction by Tumor Necrosis Factor alpha (TNF alpha), interleukin 1 (IL-1) or LPS is concentration and time dependent. [39040 ]
MAdCAM-1 is expressed on the surface of unstimulated bEnd.3 cells at early passages but not at passages greater than 30.
Intracellular adhesion molecule 1 (ICAM-1) is constitutively expressed on the cells, and expression is increased by treatment with LPS, IL-1 and TNF alpha. [39040 ]
Vascular cell adhesion molecule 1 (VCAM-1) is constitutively expressed on the cells at early passages but not at passages over 30.
P-selectin can be induced on bEnd.3 cells by Tumor Necrosis Factor alpha (TNF alpha) at both early and late passages but expression is greater at passages over 30.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0℃
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer just begins to detach. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 39039: Montesano R, et al. Increased proteolytic activity is responsible for the aberrant morphogenetic behavior of endothelial cells expressing the middle T oncogene. Cell 62: 435-445, 1990. PubMed: 2379237
39040: Sikorski EE, et al. The Peyer's patch high endothelial receptor for lymphocytes, the mucosal vascular addressin, is induced on a murine endothelial cell line by tumor necrosis factor-alpha and IL-1. J. Immunol. 151: 5239-5250, 1993. PubMed: 7693807
39041: Williams RL, et al. Embryonic lethalities and endothelial tumors in chimeric mice expressing polyoma virus middle T oncogene. Cell 52: 121-131, 1988. PubMed: 3345558