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平臺(tái)編號(hào)：bio-68130
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國(guó)際編號(hào)：ATCC CRL-1642
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細(xì)胞信息： LL/2 (LLC1)
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
LL/2 (LLC1)
生長(zhǎng)狀態(tài)：混合型生長(zhǎng)
品系：C57BL
細(xì)胞形態(tài)：其他
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：CRL-1642?
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
器官來源：肺
相關(guān)疾?。浩渌膊?br />
規(guī)格：0.5mg
Designations 統(tǒng)一別名 : LL/2 (LLC1)
Depositors:JS Bertram
Biosafety Level 生物安全等級(jí) :1
Shipped:frozen
Medium & Serum: See Propagation
Growth Properties:mixed, adherent and suspension
Organism:Mus musculus
Morphology:rounded - loosely attached or floating
Source:
Strain: C57BL
Organ: lung
Disease: Lewis lung carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.
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Applications 用途 :Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.
Tested and found negative for ectromelia virus (mousepox). The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate. This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.
Tumorigenic:Yes
Antigen
Expression:H-2b
Comments 注釋 :This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents. Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma. The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate. The cells are reported to be highly tumorigenic, but weakly metastatic in mice.
The cells form multilayers in flasks without actually becoming confluent. Tested and found negative for ectromelia virus (mousepox).
Propagation:ATCC complete growth medium:The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:fetal bovine serum to a final concentration of 10%.
Temperature 培養(yǎng)溫度 : 37.0°C
Subculturing:
Protocol: Subcultures are prepared by diluting the suspension 1:4 to 1:6.
Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature 保藏溫度 : liquid nitrogen vapor phase
Doubling Time: 21 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
References 參考文獻(xiàn) :1091: Bertram JS, Janik P. Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture. Cancer Lett. 11: 63-73, 1980. PubMed: 7226139
45212: Sharma S, et al. T cell-derived IL-10 promotes lung cancer growth by suppressing both T cell and APC function.J. Immunol. 163: 5020-5028, 1999. PubMed:10528207
