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平臺(tái)編號(hào)：bio-68144
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國(guó)際編號(hào)：CRL-2613?
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細(xì)胞信息： Cyt c-/-
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
Cyt c-/-
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
器官來(lái)源：胚胎
年限：8-9 days gestation
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：CRL-2613? Designations: Cyt c-/-
Depositors: ?RS Williams
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:epithelial
Source: Organ: embryo
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 8-9 days gestation
Comments:Murine embryos devoid of cytochrome c die in utero by midgestation. The Cyt c -/- cell line was derived from a cytochrome c null mouse embryo at E8/E9 (8-9 days gestation) prior to embryonic death. [51585 ]
Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress. [51585 ]
The Cyt c -/- cells are devoid of cytochrome c and are resistant to various types of apoptotic stimuli. [51585 ]
They show reduced caspase-3 activation and are resistant to the proapoptotic effects of UV irradiation, serum withdrawal, and staurosporine. They demonstrate increased sensitivity to cell death signals triggered by TNFalpha. [51585 ]
The Cyt c-/- cell line provides a unique resource of mammalian cells lacking cytochrome c.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with: 25 mM HEPES, 0.1 mM nonessential amino acids, an additional 2 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 0.05 mg/ml uridine, 500-1000 U/ml mouse leukemia inhibitory factor (LIF) and 20% fetal bovine serum
Temperature: 37.0°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 3 days
Preservation: Cell Culture Freezing Medium, 100% (GIBCO-BRL, Cat. No. 11101)
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 51585: Li K, et al. Cytochrome c deficiency causes embryonic lethality and attenuates stress-induced apoptosis. Cell 101: 389-399, 2000. PubMed: 10830166
數(shù)量：大量
器官來(lái)源：胚胎
年限：8-9 days gestation
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：CRL-2613? Designations: Cyt c-/-
Depositors: ?RS Williams
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:epithelial
Source: Organ: embryo
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 8-9 days gestation
Comments:Murine embryos devoid of cytochrome c die in utero by midgestation. The Cyt c -/- cell line was derived from a cytochrome c null mouse embryo at E8/E9 (8-9 days gestation) prior to embryonic death. [51585 ]
Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress. [51585 ]
The Cyt c -/- cells are devoid of cytochrome c and are resistant to various types of apoptotic stimuli. [51585 ]
They show reduced caspase-3 activation and are resistant to the proapoptotic effects of UV irradiation, serum withdrawal, and staurosporine. They demonstrate increased sensitivity to cell death signals triggered by TNFalpha. [51585 ]
The Cyt c-/- cell line provides a unique resource of mammalian cells lacking cytochrome c.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with: 25 mM HEPES, 0.1 mM nonessential amino acids, an additional 2 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 0.05 mg/ml uridine, 500-1000 U/ml mouse leukemia inhibitory factor (LIF) and 20% fetal bovine serum
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 3 days
Preservation: Cell Culture Freezing Medium, 100% (GIBCO-BRL, Cat. No. 11101)
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 51585: Li K, et al. Cytochrome c deficiency causes embryonic lethality and attenuates stress-induced apoptosis. Cell 101: 389-399, 2000. PubMed: 10830166
