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平臺(tái)編號(hào)：bio-68166
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國(guó)際編號(hào)：CRL-1764?
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細(xì)胞信息： Rat2
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
Rat2
細(xì)胞形態(tài)：成纖維樣
是否是腫瘤細(xì)胞：0
物種來源：大鼠
數(shù)量：大量
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：Fischer
年限：embryo; fetus
ATCC Number：CRL-1764?
相關(guān)疾病：正常
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5ml Designations: Rat2
Depositors: ?B Ahrens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:fibroblast
Source: Disease: normal
Strain: Fischer
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo; fetus
Comments:This line was derived from the a 5-bromo-2'-deoxyuridine resistant strain of the Fischer rat fibroblast 3T3 like cell line, Rat1 (developed by W.C. Topp).
Rat2 lacks detectable nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1835: Topp WC. Normal rat cell lines deficient in nuclear thymidine kinase. Virology 113: 408-411, 1981. PubMed: 7269249
33037: Peng M, et al. Partial inhibition of Na+/K+-ATPase by ouabain induces the Ca2+-dependent expressions of early-response genes in cardiac myocytes. J. Biol. Chem. 271: 10372-10378, 1996. PubMed: 8626609
是否是腫瘤細(xì)胞：0
物種來源：大鼠
數(shù)量：大量
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：Fischer
年限：embryo; fetus
ATCC Number：CRL-1764?
相關(guān)疾病：正常
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5ml Designations: Rat2
Depositors: ?B Ahrens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:fibroblast
Source: Disease: normal
Strain: Fischer
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo; fetus
Comments:This line was derived from the a 5-bromo-2'-deoxyuridine resistant strain of the Fischer rat fibroblast 3T3 like cell line, Rat1 (developed by W.C. Topp).
Rat2 lacks detectable nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1835: Topp WC. Normal rat cell lines deficient in nuclear thymidine kinase. Virology 113: 408-411, 1981. PubMed: 7269249
33037: Peng M, et al. Partial inhibition of Na+/K+-ATPase by ouabain induces the Ca2+-dependent expressions of early-response genes in cardiac myocytes. J. Biol. Chem. 271: 10372-10378, 1996. PubMed: 8626609
