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平臺(tái)編號(hào)：bio-68169
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國(guó)際編號(hào)：CRL-1809?
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細(xì)胞信息： Psi2 13s1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Psi2 13s1
器官來源：胚胎
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-1809?
品系：NIH/Swiss
是否是腫瘤細(xì)胞：0
物種來源：小鼠
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
規(guī)格：0.2ml Designations: Psi2 13s1
Depositors: ?RD Cone
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Cellular Products:a retrovirus vector containing the adenovirus 13S E1A and the neomycin phosphotransferase genes
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Reverse Transcript:N
HeLa Markers: N
Comments:The 13S E1A mRNA encoded by rv13S (ATCC CRL-1809 ) is 46 amino acids longer than the 12S E1A mRNA encoded by rv12S (ATCC CRL-1808 ). [21362 ]
This construct has been used to immortalize primary cells of rat brain. [21362 ]
The line was produced by inserting the E1A 13S cDNA sequence into the retrovirus vector DOL.
The resulting vector DNA (rv13S) was transfected into Psi2 cells by calcium phosphate precipitation.
The retroviruses produced during the first 24 hours post-transfection were harvested and used to infect fresh Psi2 cells to generate the Psi2 13S1 line.
The retrovirus produced by this line can be used to to immortalize primary cells of rat brain.
Unlike Psi2 12S6 (ATCC CRL 1808), only slight induction of growth is induced in liver cultures, and no proliferative response was seen with kidney, heart, pancreas adrenal, and thyroid cultures.
Tested and found negative for ectromelia virus (mousepox).
The insert contains sequences from adenovirus type 2-adenovirus type 5 hybrid cDNAs. [21362 ]
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:40 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin solution for several minutes, remove trypsin and allow the cells to sit at 37C until they begin to detach (about 3 to 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 21362: Cone RD, et al. A retrovirus expressing the 12S adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissue. Mol. Cell. Biol. 8: 1036-1044, 1988. PubMed: 2966895
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-1809?
品系：NIH/Swiss
是否是腫瘤細(xì)胞：0
物種來源：小鼠
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
規(guī)格：0.2ml Designations: Psi2 13s1
Depositors: ?RD Cone
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Cellular Products:a retrovirus vector containing the adenovirus 13S E1A and the neomycin phosphotransferase genes
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Reverse Transcript:N
HeLa Markers: N
Comments:The 13S E1A mRNA encoded by rv13S (ATCC CRL-1809 ) is 46 amino acids longer than the 12S E1A mRNA encoded by rv12S (ATCC CRL-1808 ). [21362 ]
This construct has been used to immortalize primary cells of rat brain. [21362 ]
The line was produced by inserting the E1A 13S cDNA sequence into the retrovirus vector DOL.
The resulting vector DNA (rv13S) was transfected into Psi2 cells by calcium phosphate precipitation.
The retroviruses produced during the first 24 hours post-transfection were harvested and used to infect fresh Psi2 cells to generate the Psi2 13S1 line.
The retrovirus produced by this line can be used to to immortalize primary cells of rat brain.
Unlike Psi2 12S6 (ATCC CRL 1808), only slight induction of growth is induced in liver cultures, and no proliferative response was seen with kidney, heart, pancreas adrenal, and thyroid cultures.
Tested and found negative for ectromelia virus (mousepox).
The insert contains sequences from adenovirus type 2-adenovirus type 5 hybrid cDNAs. [21362 ]
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:40 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin solution for several minutes, remove trypsin and allow the cells to sit at 37C until they begin to detach (about 3 to 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 21362: Cone RD, et al. A retrovirus expressing the 12S adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissue. Mol. Cell. Biol. 8: 1036-1044, 1988. PubMed: 2966895
