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平臺(tái)編號(hào)：bio-68182
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國(guó)際編號(hào)：CRL-8031?
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細(xì)胞信息： KG-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
KG-1
器官來(lái)源：骨髓
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
年限：59 years
ATCC Number：CRL-8031?
相關(guān)疾?。浩渌膊?br /> 運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
規(guī)格：0.2ml Designations: KG-1
Depositors: ?Genentech, Inc.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: bone marrow
Disease: acute myelogenous leukemia
Cellular Products:human leukocyte interferon
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Age: 59 years
Gender: male
Ethnicity: Caucasian
Comments:Responsive to colony stimulating activity (CSA).
Produces high levels of human leukocyte interferon upon induction by paramyxoviridae species.
See also ATCC CCL-246 .
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) viable cells/ml.
Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1056: Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682
4001: Goeddel DV. Hybrid human leukocyte interferons. US Patent 4,678,751 dated Jul 7 1987
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
年限：59 years
ATCC Number：CRL-8031?
相關(guān)疾?。浩渌膊?br /> 運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
規(guī)格：0.2ml Designations: KG-1
Depositors: ?Genentech, Inc.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: bone marrow
Disease: acute myelogenous leukemia
Cellular Products:human leukocyte interferon
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Age: 59 years
Gender: male
Ethnicity: Caucasian
Comments:Responsive to colony stimulating activity (CSA).
Produces high levels of human leukocyte interferon upon induction by paramyxoviridae species.
See also ATCC CCL-246 .
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) viable cells/ml.
Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1056: Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682
4001: Goeddel DV. Hybrid human leukocyte interferons. US Patent 4,678,751 dated Jul 7 1987
