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平臺(tái)編號(hào)：bio-68191
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國際編號(hào)：HB-152?
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細(xì)胞信息： SFR8-B6
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
SFR8-B6
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：HB-152?
是否是腫瘤細(xì)胞：0
物種來源：大鼠
規(guī)格：0.5ml Designations: SFR8-B6
Depositors: ?SF Radka
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against HLA Bw6
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with partially purified glycoprotein preparations from detergent solubilized membranes from the JY human lymphoblastoid cell line.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1002: Radka SF, et al. A monoclonal antibody directed against the HLA-BW6 epitope. J. Immunol. 128: 2804-2806, 1982. PubMed: 6176653
32497: White CA, et al. Constitutive transduction of peptide transporter and HLA genes restores antigen processing function and cytotoxic T cell-mediated immune recognition of human melanoma cells. Int. J. Cancer 75: 590-595, 1998. PubMed: 9466661
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：HB-152?
是否是腫瘤細(xì)胞：0
物種來源：大鼠
規(guī)格：0.5ml Designations: SFR8-B6
Depositors: ?SF Radka
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against HLA Bw6
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with partially purified glycoprotein preparations from detergent solubilized membranes from the JY human lymphoblastoid cell line.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1002: Radka SF, et al. A monoclonal antibody directed against the HLA-BW6 epitope. J. Immunol. 128: 2804-2806, 1982. PubMed: 6176653
32497: White CA, et al. Constitutive transduction of peptide transporter and HLA genes restores antigen processing function and cytotoxic T cell-mediated immune recognition of human melanoma cells. Int. J. Cancer 75: 590-595, 1998. PubMed: 9466661
