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S6F1
    S6F1
  • 平臺編號:bio-68195
  • 國際編號:HB-9579?
  • 細(xì)胞信息: S6F1
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

生長狀態(tài):懸浮生長
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG1
是否是腫瘤細(xì)胞:0
物種來源:小鼠
ATCC Number:HB-9579?
運(yùn)輸方式:凍存運(yùn)輸
數(shù)量:大量
規(guī)格:0.5ml Designations: S6F1
Depositors: ?Dana Farber Cancer Inst.
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against a subset of human CD8 (LFA-1) positive T cells
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody recognizes a subset of CD8 positive T cells that have cytotoxic effector activity.
The S6F1 epitope is absent on CD8 positive suppressor cells.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 0.1 mM hypoxanthine, and 0.032 mM thymidine, 80%; heat-inactivated fetal bovine serum, 10%; heat-inactivated horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21964: Schlossman SF, Morimoto C. Monoclonal antibody specific to a novel epitope of the LFA-1 antigen of human T lymphocytes. US Patent 5,002,869 dated Mar 26 1991

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