-
平臺(tái)編號(hào)：bio-68197
-
國際編號(hào)：HB-213?
-
細(xì)胞信息： CDR1
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費(fèi)用：
加載中…… - 訂購
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
CDR1
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2a
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：大鼠
生長狀態(tài)：懸浮生長
ATCC Number：HB-213?
規(guī)格：0.1ml Designations: CDR1
Depositors: ?RV Rouse
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse cortical thymic epithelium and a 160000 dalton Abelson leukemia antigen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with mouse thymic stromal tissue.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody does not react with the thymic medulla, and the reactivity appears to be identical to that reported for the 6C3 hybridoma (Proc. Natl. Acad. Sci. USA 81:4434-4438, 1984).
It does not react with rat or human thymus, but does cross-reacts with epithelial elements in mouse kidney, large and small intestine, brain and spleen vasculature, and human skin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 26303: Pillemer E, et al. Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products. Proc. Natl. Acad. Sci. USA 81: 4434-4438, 1984. PubMed: 6611551
58334: Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2a
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：大鼠
生長狀態(tài)：懸浮生長
ATCC Number：HB-213?
規(guī)格：0.1ml Designations: CDR1
Depositors: ?RV Rouse
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse cortical thymic epithelium and a 160000 dalton Abelson leukemia antigen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with mouse thymic stromal tissue.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody does not react with the thymic medulla, and the reactivity appears to be identical to that reported for the 6C3 hybridoma (Proc. Natl. Acad. Sci. USA 81:4434-4438, 1984).
It does not react with rat or human thymus, but does cross-reacts with epithelial elements in mouse kidney, large and small intestine, brain and spleen vasculature, and human skin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 26303: Pillemer E, et al. Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products. Proc. Natl. Acad. Sci. USA 81: 4434-4438, 1984. PubMed: 6611551
58334: Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413
