ATCC Number:TIB-154?
數(shù)量:大量
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG3
運(yùn)輸方式:凍存運(yùn)輸
是否是腫瘤細(xì)胞:0
物種來源:小鼠
生長狀態(tài):懸浮生長
規(guī)格:0.1ml Designations: BP107.2.2
Depositors: ?F Symington
Isotype: IgG3
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against I-A b and I-A d; cytotoxic
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Comments:Animals were immunized with Con A stimulated B10.D2 lymph node lymphocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody cross-reacts with the p, q, u, j and v haplotypes.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 941: Sprent J, Symington FW. A monoclonal antibody detecting an Ia specificity mapping in the I-A or I-E subregion. Immunogenetics 14: 53-61, 1981. PubMed: 6173320