-
平臺編號：bio-68204
-
國際編號：CRL-2492?
-
細(xì)胞信息： 44-26-2
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費(fèi)用：
加載中…… - 訂購
-
注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
44-26-2
是否是腫瘤細(xì)胞：0
物種來源：小鼠
細(xì)胞形態(tài)：淋巴樣
免疫類型：mouse IgG1
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
生長狀態(tài)：懸浮生長
ATCC Number：CRL-2492?
規(guī)格：20T Designations: 44-26-2
Depositors: ?DG Sieckmann
Isotype: mouse IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: lymphoblast B lymphocyte; somatic cell hybrid
Cellular Products:immunoglobulin; monoclonal antibody; against an idiotypic determinant on 1D2 (anti-ricin A chain monoclonal antibody)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.
Isolation: Isolation date: 1995
Comments:Animals were immunized against 1D2, a BALB/cJ mouse IgG1 monoclonal antibody specific for ricin A chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The 44-6-1 (ATCC CRL-2491 ) and 44-26-2 (ATCC CRL-2492 ) cell lines produce antibodies that bind specifically to an idiotypic determinant on 1D2 anti-ricin monoclonal antibody.
The 44-6-1 and 44-26-2 monoclonals conjugated to keyhole limpet hemocyanin (KLH) have been shown to induce anti-ricin antibodies in mice.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 1 x 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 49767: Sieckmann DG, et al. Development of an anti-idiotypic vaccine for ricin. J. Allergy Clin. Immunol. 99: S286, 1997.
物種來源：小鼠
細(xì)胞形態(tài)：淋巴樣
免疫類型：mouse IgG1
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
生長狀態(tài)：懸浮生長
ATCC Number：CRL-2492?
規(guī)格：20T Designations: 44-26-2
Depositors: ?DG Sieckmann
Isotype: mouse IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: lymphoblast B lymphocyte; somatic cell hybrid
Cellular Products:immunoglobulin; monoclonal antibody; against an idiotypic determinant on 1D2 (anti-ricin A chain monoclonal antibody)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.
Isolation: Isolation date: 1995
Comments:Animals were immunized against 1D2, a BALB/cJ mouse IgG1 monoclonal antibody specific for ricin A chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The 44-6-1 (ATCC CRL-2491 ) and 44-26-2 (ATCC CRL-2492 ) cell lines produce antibodies that bind specifically to an idiotypic determinant on 1D2 anti-ricin monoclonal antibody.
The 44-6-1 and 44-26-2 monoclonals conjugated to keyhole limpet hemocyanin (KLH) have been shown to induce anti-ricin antibodies in mice.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 1 x 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 49767: Sieckmann DG, et al. Development of an anti-idiotypic vaccine for ricin. J. Allergy Clin. Immunol. 99: S286, 1997.
