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數(shù)量：大量
細(xì)胞形態(tài)：內(nèi)皮樣
ATCC Number：CCL-209?
相關(guān)疾?。赫?br /> 運輸方式：凍存運輸
生長狀態(tài)：貼壁生長
細(xì)胞類型：內(nèi)皮細(xì)胞
器官來源：其他
是否是腫瘤細(xì)胞：0
物種來源：牛
組織來源：vascular endothelium
規(guī)格：0.1ml Designations: CPAE
Depositors: ?PJ Del Vecchio
Biosafety Level:2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Bos taurus
Morphology:endothelial


Source: Organ: pulmonary artery
Tissue: vascular endothelium
Disease: normal
Cell Type: endothelial
Cellular Products:positive for angiotensin converting enzyme
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis:normal female; diploid; stable
Gender: female
Comments:CPAE is an endothelial cell line derived from the main stem pulmonary artery of a young cow (Bos taurus). This line was initiated in January, 1979 by P. Del Vecchio from artery lumen scrapings dispersed by enzyme treatment.
Subsequent to the publication of the sixth edition of the ATCC catalog, tests for bovine diarrhea virus (BVD) have indicated that CPAE cells test positive for BVD viral antigen. [22883 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
33004: Girard JP, Springer TA. Modulation of endothelial cell adhesion by hevin, an acidic protein associated with high endothelial venules. J. Biol. Chem. 271: 4511-4517, 1996. PubMed: 8626806