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平臺編號：bio-68220
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國際編號：CRL-2384?
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細胞信息： anti-SRp20 (7B4)
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
anti-SRp20 (7B4)
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：CRL-2384?
細胞形態(tài)：淋巴樣
免疫類型：IgG1
數(shù)量：大量
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
規(guī)格：0.5mg Designations: anti-SRp20 (7B4)
Depositors: ?KM Neugebauer, MB Roth
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against SRp20 proteins (pre-mRNA splicing factors)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with a peptide synthesized to correspond to amino acids 84 to 104 of the predicted protein that occur between the RNA recognition motif and the SR domain.
Spleen cells were fused with FOX-NY myeloma cells.
The antibody reacts with a single member, SRp20, of the family of essential splicing factors the SR proteins.
SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins).
The family consists of at least 6 proteins with approximate masses of 75000, 55000, 40000, two at 30000 and 20000 daltons.
The antibody is used to determine if a factor in RNA metabolism is SRp20.
The antibody is useful for immunoprecipitation, immunoblotting and immunocytochemistry assays
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.075 mM adenine, 800 nM aminopterin, 0.016 mM thymidine (AAT) and 20% fetal bovine serum
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternately, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
References: 39770: Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396
物種來源：小鼠
ATCC Number：CRL-2384?
細胞形態(tài)：淋巴樣
免疫類型：IgG1
數(shù)量：大量
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
規(guī)格：0.5mg Designations: anti-SRp20 (7B4)
Depositors: ?KM Neugebauer, MB Roth
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against SRp20 proteins (pre-mRNA splicing factors)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with a peptide synthesized to correspond to amino acids 84 to 104 of the predicted protein that occur between the RNA recognition motif and the SR domain.
Spleen cells were fused with FOX-NY myeloma cells.
The antibody reacts with a single member, SRp20, of the family of essential splicing factors the SR proteins.
SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins).
The family consists of at least 6 proteins with approximate masses of 75000, 55000, 40000, two at 30000 and 20000 daltons.
The antibody is used to determine if a factor in RNA metabolism is SRp20.
The antibody is useful for immunoprecipitation, immunoblotting and immunocytochemistry assays
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.075 mM adenine, 800 nM aminopterin, 0.016 mM thymidine (AAT) and 20% fetal bovine serum
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternately, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
References: 39770: Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396
