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生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
數量：大量
細胞類型：B淋巴細胞
ATCC Number：CCL-120?
相關疾病：白血病
是否是腫瘤細胞：0
物種來源：人
運輸方式：凍存運輸
年限：11.5 years
器官來源：外周血
規(guī)格：0.1ml Designations: CCRF-SB [CCRF SB]
Depositors: ?GE Foley
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast


Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: B lymphoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:HLA A1, A2, B12, B17, Cw2
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18
Isoenzymes: G6PD, B
Age: 11.5 years
Gender: male
Ethnicity: Caucasian
Comments:A T cell line (HSB-2, ATCC CCL-120.1 ) was also obtained from the same buffy coat preparation.
Derived from a buffy coat preparation from a patient with ALL.
The cells are EBNA positive.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days
Related Products:derived from same individual:ATCC CCL-120.1
References: 24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967.
25967: Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381