ATCC Number:CCL-55?
器官來源:其他
細胞形態(tài):成纖維樣
生長狀態(tài):貼壁生長
數(shù)量:大量
運輸方式:凍存運輸
是否是腫瘤細胞:0
物種來源:其他
規(guī)格:48T Designations: RTG-2
Depositors: ?K Wolf
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Oncorhynchus mykiss deposited as Salmo gairdneri
Morphology:fibroblast
Source: Organ: mixed; testis; ovary
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 1
Gender: male and female mixed
Comments:Maximum growth rate occurs at 22C.
Above 26C the cells do not survive.
Cells will grow at temperatures as low as 4C.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Once per week
Harvest cells with cold (less than 24C) 0.25% trypsin or 0.02% versene (or a mixture of the two). Cultures may be established with as few as 3 X 10 exp3 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22297: . . Science 135: 1065, 1962.
25973: Officer JE. Ability of a fish cell line to support the growth of mammalian viruses. Proc. Soc. Exp. Biol. Med. 116: 190-194, 1964. PubMed: 14200103
53245: Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741