細(xì)胞形態(tài):淋巴樣
免疫類型:IgM
品系:BALB/c
是否是腫瘤細(xì)胞:0
物種來源:小鼠
運(yùn)輸方式:凍存運(yùn)輸
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
數(shù)量:大量
器官來源:脾
ATCC Number:HB-8678?
規(guī)格:48T Designations: 7C2C5C12
Depositors: ?USDA
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: spleen
Strain: BALB/c
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Trichinella spiralis
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Tumorigenic:Yes
Comments:Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
References: 58020: Gamble HR, Graham CE. Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. Am. J. Vet. Res. 45: 67-74, 1984. PubMed: 6703457