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平臺(tái)編號(hào)：bio-68228
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國(guó)際編號(hào)：CRL-2204?
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細(xì)胞信息： PA317 LXSN 16E6
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
PA317 LXSN 16E6
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
年限：embryo
器官來(lái)源：胚胎
品系：NIH/Swiss
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：CRL-2204?
細(xì)胞形態(tài)：成纖維樣
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
規(guī)格：0.2ml Designations: PA317 LXSN 16E6
Depositors: ?DA Galloway
Biosafety Level:2 [Cells contain human papilloma viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Note: The user of the PA317 LXSN 16E6 cell line agrees to indemnify and hold harmless the United States, the ATCC , Denise A. Galloway and Fred Hutchinson Cancer Research Center, Seattle, Washington from any claims, costs, damages, or expenses resulting from any injury (including death), damage or loss that may arise from the use of the cell line either directly (including use for diagnostic purposes) or in the preparation of a product. The user assumes all risks and responsibilities in connection with the receipt, handling, storage and use of the material.
Age: embryo
Comments:PA317 LXSN 16E6 is a packaging cell line developed by transfection of the retrovirus vector pLXSN16E6 into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
The pLXSN16E6 vector contains the human papilloma virus (HPV) type 16 E6 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter- enhancer sequences.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line produces the amphotropic retrovirus LXSN16E6 which encodes the HPV16 E6 open reading frame, and which can be used to stably infect many cell types.
The resulting cells constitutively express the E6 protein of HPV16 which targets p53 for degradation.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902
物種來(lái)源：小鼠
年限：embryo
器官來(lái)源：胚胎
品系：NIH/Swiss
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：CRL-2204?
細(xì)胞形態(tài)：成纖維樣
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
規(guī)格：0.2ml Designations: PA317 LXSN 16E6
Depositors: ?DA Galloway
Biosafety Level:2 [Cells contain human papilloma viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Note: The user of the PA317 LXSN 16E6 cell line agrees to indemnify and hold harmless the United States, the ATCC , Denise A. Galloway and Fred Hutchinson Cancer Research Center, Seattle, Washington from any claims, costs, damages, or expenses resulting from any injury (including death), damage or loss that may arise from the use of the cell line either directly (including use for diagnostic purposes) or in the preparation of a product. The user assumes all risks and responsibilities in connection with the receipt, handling, storage and use of the material.
Age: embryo
Comments:PA317 LXSN 16E6 is a packaging cell line developed by transfection of the retrovirus vector pLXSN16E6 into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
The pLXSN16E6 vector contains the human papilloma virus (HPV) type 16 E6 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter- enhancer sequences.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line produces the amphotropic retrovirus LXSN16E6 which encodes the HPV16 E6 open reading frame, and which can be used to stably infect many cell types.
The resulting cells constitutively express the E6 protein of HPV16 which targets p53 for degradation.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902
