運輸方式:凍存運輸
數(shù)量:大量
生長狀態(tài):懸浮生長
是否是腫瘤細(xì)胞:0
物種來源:大鼠
ATCC Number:TIB-183?
細(xì)胞形態(tài):淋巴樣
免疫類型:IgM
規(guī)格:0.3ml Designations: J11d.2
Depositors: ?J Sprent
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse cortical thymocytes, erythrocytes, neutrophils and B lymphocytes (B cells); cytotoxic
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Comments:Animals were immunized with C3H/HeJ thymocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not react with mature (medullary) thymocytes, pluripotential stem cells, platelets or cells of monocyte-macrophage lineage.
IgG antibody producing cells are strongly positive for the J11d.2 antigen.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 975: Bruce J, et al. A monoclonal antibody discriminating between subsets of T and B cells. J. Immunol. 127: 2496-2501, 1981. PubMed: 6975326