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J11d.2
    J11d.2
  • 平臺編號:bio-68232
  • 國際編號:TIB-183?
  • 細(xì)胞信息: J11d.2
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

運輸方式:凍存運輸
數(shù)量:大量
生長狀態(tài):懸浮生長
是否是腫瘤細(xì)胞:0
物種來源:大鼠
ATCC Number:TIB-183?
細(xì)胞形態(tài):淋巴樣
免疫類型:IgM
規(guī)格:0.3ml Designations: J11d.2
Depositors: ?J Sprent
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse cortical thymocytes, erythrocytes, neutrophils and B lymphocytes (B cells); cytotoxic
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with C3H/HeJ thymocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not react with mature (medullary) thymocytes, pluripotential stem cells, platelets or cells of monocyte-macrophage lineage.
IgG antibody producing cells are strongly positive for the J11d.2 antigen.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 975: Bruce J, et al. A monoclonal antibody discriminating between subsets of T and B cells. J. Immunol. 127: 2496-2501, 1981. PubMed: 6975326

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