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平臺(tái)編號(hào)：bio-68238
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國(guó)際編號(hào)：CRL-2199?
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細(xì)胞信息： C6/LacZ
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
C6/LacZ
數(shù)量：大量
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：CRL-2199?
相關(guān)疾?。荷窠?jīng)膠質(zhì)瘤
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
器官來源：大腦
是否是腫瘤細(xì)胞：0
物種來源：大鼠
品系：outbred
細(xì)胞形態(tài)：成纖維樣
規(guī)格：0.1ml Designations: C6/LacZ
Depositors: ?LA Lampson
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:fibroblast
Source: Organ: brain
Strain: outbred
Disease: glioma
Cell Type: glial cell;
Cellular Products:beta galactosidase (beta-gal)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:The C6/lacZ cell line was developed in 1989 from the C6 cell line (N-nitrosomethylurea induced rat glial tumor).
C6 cells were infected with the BAG replication deficient retroviral vector carrying the E. coli lacZ gene encoding beta-gal and the Tn5 neomycin gene, which confers resistance to G418.
The cells were cultured in G418 for 14 days, cloned, and evaluated for beta-gal production.
The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified.
Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide.
The contrast between stained cells and background facilitates image analysis.
This is one of few models that permit quantitative analysis of microscopic tumor in the brain.
The tumor mimics important features of human brain tumor growth and spread.
The growth pattern of 6C/lacZ complements that of 9L/lacZ (see ATCC CRL-2200 ). C6/lacZ, but not 9L/lacZ, shows infiltrative growth in the brain.
The beta-gal expression is less stable than for 9L/lacZ, and the cells should be used soon after thawing or re-cloned.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, and 1 mM sodium pyruvate 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, rinse with PBS and add fresh 0.25% trypsin, 0.03% EDTA solution and incubate at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:derivative:ATCC CRL-2303
References: 22438: Lampson LA, et al. Exploiting the lacZ reporter gene for quantitative analysis of disseminated tumor growth within the brain: use of the lacZ gene product as a tumor antigen, for evaluation of antigenic modulation, and to facilitate image analysis of tumor growth in situ. Cancer Res. 53: 176-182, 1993. PubMed: 8416743
23091: Lampson LA, et al. Disseminating tumor cells and their interactions with leukocytes visualized in the brain. Cancer Res. 52: 1018-1025, 1992. PubMed: 1737331
24411: Lampson LA, et al. A new model permits study of the immune response to individual tumor cells within the brain. Neurology 40: 396-397, 1990.
90374: Dutta T, et al. Robust ability of IFN-gamma to upregulate class II MHC antigen expression in tumor bearing rat brains. J. Neuro-Oncol. 64: 31-44, 2003. PubMed: 12952284
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：CRL-2199?
相關(guān)疾?。荷窠?jīng)膠質(zhì)瘤
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
器官來源：大腦
是否是腫瘤細(xì)胞：0
物種來源：大鼠
品系：outbred
細(xì)胞形態(tài)：成纖維樣
規(guī)格：0.1ml Designations: C6/LacZ
Depositors: ?LA Lampson
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:fibroblast
Source: Organ: brain
Strain: outbred
Disease: glioma
Cell Type: glial cell;
Cellular Products:beta galactosidase (beta-gal)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:The C6/lacZ cell line was developed in 1989 from the C6 cell line (N-nitrosomethylurea induced rat glial tumor).
C6 cells were infected with the BAG replication deficient retroviral vector carrying the E. coli lacZ gene encoding beta-gal and the Tn5 neomycin gene, which confers resistance to G418.
The cells were cultured in G418 for 14 days, cloned, and evaluated for beta-gal production.
The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified.
Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide.
The contrast between stained cells and background facilitates image analysis.
This is one of few models that permit quantitative analysis of microscopic tumor in the brain.
The tumor mimics important features of human brain tumor growth and spread.
The growth pattern of 6C/lacZ complements that of 9L/lacZ (see ATCC CRL-2200 ). C6/lacZ, but not 9L/lacZ, shows infiltrative growth in the brain.
The beta-gal expression is less stable than for 9L/lacZ, and the cells should be used soon after thawing or re-cloned.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, and 1 mM sodium pyruvate 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, rinse with PBS and add fresh 0.25% trypsin, 0.03% EDTA solution and incubate at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:derivative:ATCC CRL-2303
References: 22438: Lampson LA, et al. Exploiting the lacZ reporter gene for quantitative analysis of disseminated tumor growth within the brain: use of the lacZ gene product as a tumor antigen, for evaluation of antigenic modulation, and to facilitate image analysis of tumor growth in situ. Cancer Res. 53: 176-182, 1993. PubMed: 8416743
23091: Lampson LA, et al. Disseminating tumor cells and their interactions with leukocytes visualized in the brain. Cancer Res. 52: 1018-1025, 1992. PubMed: 1737331
24411: Lampson LA, et al. A new model permits study of the immune response to individual tumor cells within the brain. Neurology 40: 396-397, 1990.
90374: Dutta T, et al. Robust ability of IFN-gamma to upregulate class II MHC antigen expression in tumor bearing rat brains. J. Neuro-Oncol. 64: 31-44, 2003. PubMed: 12952284
