-
平臺編號：bio-68240
-
國際編號：ATCC TIB-215
-
細(xì)胞信息： SKW 6.4
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費用：
加載中…… - 訂購
-
注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
SKW 6.4
運輸方式：凍存運輸
ATCC Number：TIB-215
生長狀態(tài)：懸浮生長
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
規(guī)格：1mg Designations 拉丁名 : SKW 6.4
Depositors:P Ralph
Isotype: IgM
Biosafety Level 生物安全等級 :2 [Cells Contain HERPESVIRUS ]
Shipped 提供形式 :frozen
Medium Serum: See Propagation
Growth Properties:suspension
Organism:Homo sapiens
Morphology:lymphoblast
Source:
Cell Type:Blymphocyte; Epstein-Barr virus (EBV) transformed
Cellular Products:immunoglobulin; monoclonal antibody
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.
Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.
Please click here for information regarding the specific requirements for shipment to your location.DNA Profile (STR):Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 THO1: 8,9.3 TPOX: 8,11 vWA: 17,20
Comments 注釋 :The cells respond to T Cell Replacing Factor (TRF) and B Cell Differentiation Factor (BCDF) by secreting IgM.
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium,Catalog No. 30-2001.
To make the complete growth medium, add he following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature 培養(yǎng)溫度 : 37.0°C
Subculturing:Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 5 x 10 exp5 cells/ml and maintain between 4 x 10 exp5 and 2 x 10 exp6 cells/ml.
Preservation:culture medium 95%; DMSO, 5% Related
Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020
References 參考文獻(xiàn) :1149: Saiki O, Ralph P. Clonal differences in response to T cell replacing factor (TRF) for IgM secretion and TRF receptors in a human B lymphoblast cell line. Eur. J.Immunol. 13: 31-34, 1983. PubMed: 6339249
58055: Ralph P, et al. IgM and IgG secretion in human B-cell lines regulated by B-cell-inducing factors (BIF) and phorbol ester.Immunol. Lett. 7: 17-23,1983. PubMed: 6605913
