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平臺(tái)編號(hào)：bio-68241
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國(guó)際編號(hào)：CRL-1709?
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細(xì)胞信息： MOP-8
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
MOP-8
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
器官來(lái)源：胚胎
品系：NIH/Swiss
年限：embryo
ATCC Number：CRL-1709?
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞類(lèi)型：SV40轉(zhuǎn)化細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
規(guī)格：0.2ml Designations: MOP-8
Depositors: ?JA Hassell
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Strain: NIH/Swiss
Organ: embryo
Cell Type: SV40 transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Transfection host, especially for plasmids containing the polyomavirus origin of replication. [9861 ]
Age: embryo
Comments:The MOP-8 cell line was derived by transforming NIH 3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus.
The cells will support the replication of recombinant plasmids which contain the polyomavirus origin for DNA synthesis.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 9861: Muller WJ, et al. Isolation of large T antigen-producing mouse cell lines capable of supporting replication of polyomavirus-plasmid recombinants. Mol. Cell. Biol. 4: 2406-2412, 1984. PubMed: 6096696
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