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平臺編號：bio-68244
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國際編號：CRL-1656?
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細胞信息： Mpf
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Mpf
器官來源：大腦
生長狀態(tài)：貼壁生長
是否是腫瘤細胞：0
物種來源：其他
ATCC Number：CRL-1656?
運輸方式：凍存運輸
相關(guān)疾?。赫?br /> 年限：6 weeks
數(shù)量：大量
規(guī)格：0.2ml Designations: Mpf
Depositors: ?RS Trowbridge
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mustela putoris furo
Morphology:
Source: Organ: brain
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Age: 6 weeks
Comments:Mpf cells have an absolute cloning efficiency greater than 45%.
Propagation: ATCC complete growth medium: Basal medium (Eagle) in Earle's BSS, 86%; lamb serum, 14%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Doubling Time: 12 to 13 hrs
References: 22668: Trowbridge RS, et al. Establishment and characterization of ferret cells in culture. In Vitro 18: 952-960, 1982. PubMed: 6185411
生長狀態(tài)：貼壁生長
是否是腫瘤細胞：0
物種來源：其他
ATCC Number：CRL-1656?
運輸方式：凍存運輸
相關(guān)疾?。赫?br /> 年限：6 weeks
數(shù)量：大量
規(guī)格：0.2ml Designations: Mpf
Depositors: ?RS Trowbridge
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mustela putoris furo
Morphology:
Source: Organ: brain
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Age: 6 weeks
Comments:Mpf cells have an absolute cloning efficiency greater than 45%.
Propagation: ATCC complete growth medium: Basal medium (Eagle) in Earle's BSS, 86%; lamb serum, 14%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Doubling Time: 12 to 13 hrs
References: 22668: Trowbridge RS, et al. Establishment and characterization of ferret cells in culture. In Vitro 18: 952-960, 1982. PubMed: 6185411
