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平臺(tái)編號(hào)：bio-68252
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國(guó)際編號(hào)：ATCC CRL-2502
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細(xì)胞信息： ARPE-19/HPV-16
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu) 說(shuō)明書(shū)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
ARPE-19/HPV-16
器官來(lái)源：眼
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：CRL-2502
細(xì)胞形態(tài)：上皮樣
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
組織來(lái)源：retinal pigmented epithelium; retina
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
數(shù)量：大量
年限：19 years
規(guī)格：0.5mg Designations 統(tǒng)一別名 : ARPE-19/HPV-16
Depositors:JW Obringer
Biosafety Level 生物安全等級(jí) :2 [Cells contain Human Papilloma viral 16 (HPV16) sequences ]
Shipped 提供形式 :frozen
Medium Serum: See Propagation
Growth
Properties:adherent
Organism:Homo sapiens deposited as human
Morphology:epithelial
Source:
Organ: eye
Tissue: retinal pigmented epithelium; retina
Cell Type: human papillomavirus 16 (HPV-16) transfected
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.
Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.
Please click here for information regarding the specific requirements for shipment to your location.
Applications 用途 :transfection host (Roche Transfection Reagents )
Antigen
Expression:RPE-specific markers CRALBP and RPE-65 [34497 ] DNA
Profile (STR):Amelogenin: X, Y CSF1PO: 11
D13S317: 11, 12 D16S539: 9, 11 D5S818: 13
D7S820: 9, 11 THO1: 6, 9.3 TPOX: 9, 11
vWA: 16, 19
Cytogenetic
Analysis:diploid
Age:19 years
Gender:male
Comments 注釋 :The ARPE/HPV-16 transformed cell line was derived from the ARPE-19 cell line (ATCC CRL-2302 ) by transfection with DH5-HPV-16. ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident.
[34497 ] The cells express the RPE-specific markers CRALBP and RPE-65.
[34497 ] Propagation:ATCC complete growthmedium: The base medium for this cell line is ATCC-formulated
DMEM:F12 Medium Catalog No. 30-2006.
To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature 培養(yǎng)溫度 : 37.0°C
Subculturing:Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting.
Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium.
Add appropriate aliquots of the cell suspension to culture vessels. Incubate cultures at 37C.
Preservation:culture medium 95%; DMSO, 5%
Related
Products:recommended serum:ATCC 30-2020 parental cell line:ATCC CRL-2302
References 參考文獻(xiàn) :34497: Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties.Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076
