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平臺編號：bio-68260
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國際編號：CRL-1456?
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細(xì)胞信息： G-8
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
G-8
生長狀態(tài)：貼壁生長
組織來源：skeletal muscle
細(xì)胞形態(tài)：成肌細(xì)胞
年限：fetus fetus
運輸方式：凍存運輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：CRL-1456?
數(shù)量：大量
規(guī)格：0.5mg Designations: G-8
Depositors: ?J Peacock
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:myoblast
Source: Tissue: skeletal muscle
Cell Type: myoblast myoblast;
Cellular Products:myosin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:acetylcholine (The cells are highly responsive to acetylcholine.)
Tumorigenic:No
Cytogenetic Analysis:modal number = 66
Age: fetus fetus
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks.Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Doubling Time: 25 to 27 hrs
References: 1063: Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191
組織來源：skeletal muscle
細(xì)胞形態(tài)：成肌細(xì)胞
年限：fetus fetus
運輸方式：凍存運輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：CRL-1456?
數(shù)量：大量
規(guī)格：0.5mg Designations: G-8
Depositors: ?J Peacock
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:myoblast
Source: Tissue: skeletal muscle
Cell Type: myoblast myoblast;
Cellular Products:myosin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:acetylcholine (The cells are highly responsive to acetylcholine.)
Tumorigenic:No
Cytogenetic Analysis:modal number = 66
Age: fetus fetus
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks.Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Doubling Time: 25 to 27 hrs
References: 1063: Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191
