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平臺(tái)編號(hào)：bio-68267
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國(guó)際編號(hào)：CRL-1780?
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細(xì)胞信息： RF/6A
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
RF/6A
年限：fetus
數(shù)量：大量
ATCC Number：CRL-1780?
相關(guān)疾?。赫?br /> 細(xì)胞形態(tài)：上皮樣
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞類(lèi)型：內(nèi)皮細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：獼猴
組織來(lái)源：choroid; retina
規(guī)格：0.5ml Designations: RF/6A
Depositors: ?F Hu
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Macaca mulatta
Morphology:epithelial
Source: Tissue: choroid; retina
Disease: normal
Cell Type: endothelial
Cellular Products:factor VIII; fibronectin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
Age: fetus
Comments:This line spontaneously transformed at an early age and has been passaged over 540 times.
Weibel-Palade granules are seen by electron microscopy.
Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice weekly
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22315: Lou DA, Hu FN. Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454
數(shù)量：大量
ATCC Number：CRL-1780?
相關(guān)疾?。赫?br /> 細(xì)胞形態(tài)：上皮樣
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞類(lèi)型：內(nèi)皮細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：獼猴
組織來(lái)源：choroid; retina
規(guī)格：0.5ml Designations: RF/6A
Depositors: ?F Hu
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Macaca mulatta
Morphology:epithelial
Source: Tissue: choroid; retina
Disease: normal
Cell Type: endothelial
Cellular Products:factor VIII; fibronectin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
Age: fetus
Comments:This line spontaneously transformed at an early age and has been passaged over 540 times.
Weibel-Palade granules are seen by electron microscopy.
Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice weekly
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22315: Lou DA, Hu FN. Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454
