細胞類型:成纖維細胞
細胞形態(tài):成纖維樣
是否是腫瘤細胞:0
物種來源:人
數(shù)量:大量
ATCC Number:CCL-210?
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運輸方式:凍存運輸
器官來源:肺
生長狀態(tài):貼壁生長
年限:20 years
規(guī)格:0.1ml Designations: CCD-19Lu
Depositors: ?S Dilworth
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11
D5S818: 12
D7S820: 9,11
THO1: 9.3
TPOX: 8
vWA: 17,18
Cytogenetic Analysis:normal female; diploid; stable
Age: 20 years
Gender: female
Ethnicity: Caucasian
Comments:The line was derived from normal tissue from patient who died of trauma to the head.
The cells are capable of at least 8 or 9 population doublings beyond the current freeze.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for two minutes at room temperature. Remove trypsin and let the culture stand at 37C for five minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.