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平臺(tái)編號(hào)：bio-68272
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國(guó)際編號(hào)：CRL-1621?
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細(xì)胞信息： ARH-77
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
ARH-77
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
年限：33 years
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1; kappa light chain
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：外周血
ATCC Number：CRL-1621?
相關(guān)疾?。浩渌膊?br /> 規(guī)格：0.5mg Designations: ARH-77
Depositors: ?BD Drewinko
Isotype: IgG1; kappa light chain
Biosafety Level:2 [Herpesvirus; cells contain EBV ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: plasma cell leukemia
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD11a +; CD19 +; CD20 +; CD28 +; CD38 -; CD49e +
DNA Profile (STR):Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8,9.3
TPOX: 8
vWA: 17
Age: 33 years
Gender: female
Ethnicity: Caucasian
Comments:The ARH-77 cell line was established from the peripheral blood of a patient suffering from IgG plasma cell leukemia.
Although established from cells taken from a patient with a plasma cell leukemia, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270 ]
The cells are positive for Epstein-Barr nuclear antigen(EBNA +) and Epstein-Barr viral capsid antigen (EBVCA +). [58044 ]
The line has been cured of a previous mycoplasma infection.
LICR-LON-HMy2 (HMy2), a fast growing mutant of the ARH-77 cell line (ATCC CRL-1621 ), was irradiated and selected for class I loss to establish the HMy2.C1R (ATCC CRL-1993) cell line. [26111 ]
Subsequently, C1R-neo (ATCC CRL-2369 ), C1R-sB7 (ATCC CRL-2370 ), and C1R-B7 (ATCC CRL-2371 ) were established by transfection of the HMy2.C1R cell line. [23312 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen apor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1993
References: 1089: Burk KH, et al. Establishment of a human plasma cell line in vitro. Cancer Res. 38: 2508-2513, 1978. PubMed: 566614
1651: Cote RJ, et al. Generation of human monoclonal antibodies reactive with cellular antigens. Proc. Natl. Acad. Sci. USA 80: 2026-2030, 1983. PubMed: 6572959
23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569
26111: Edwards PA, et al. A human-hybridoma system based on a fast-growing mutant of the ARH-77 plasma cell leukemia-derived line. Eur. J. Immunol. 12: 641-648, 1982. PubMed: 7140810
26112: . . Proc. Am. Assoc. Cancer Res. 19: 211, 1978.
43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762
58044: Drewinko B, et al. ARH-77, an established human IgG-producing myeloma cell line. I. Morphology, B-cell phenotypic marker profile, and expression of Epstein-Barr virus. Cancer 54: 1883-1892, 1984. PubMed: 6090003
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
年限：33 years
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1; kappa light chain
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：外周血
ATCC Number：CRL-1621?
相關(guān)疾?。浩渌膊?br /> 規(guī)格：0.5mg Designations: ARH-77
Depositors: ?BD Drewinko
Isotype: IgG1; kappa light chain
Biosafety Level:2 [Herpesvirus; cells contain EBV ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: plasma cell leukemia
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD11a +; CD19 +; CD20 +; CD28 +; CD38 -; CD49e +
DNA Profile (STR):Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8,9.3
TPOX: 8
vWA: 17
Age: 33 years
Gender: female
Ethnicity: Caucasian
Comments:The ARH-77 cell line was established from the peripheral blood of a patient suffering from IgG plasma cell leukemia.
Although established from cells taken from a patient with a plasma cell leukemia, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270 ]
The cells are positive for Epstein-Barr nuclear antigen(EBNA +) and Epstein-Barr viral capsid antigen (EBVCA +). [58044 ]
The line has been cured of a previous mycoplasma infection.
LICR-LON-HMy2 (HMy2), a fast growing mutant of the ARH-77 cell line (ATCC CRL-1621 ), was irradiated and selected for class I loss to establish the HMy2.C1R (ATCC CRL-1993) cell line. [26111 ]
Subsequently, C1R-neo (ATCC CRL-2369 ), C1R-sB7 (ATCC CRL-2370 ), and C1R-B7 (ATCC CRL-2371 ) were established by transfection of the HMy2.C1R cell line. [23312 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen apor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1993
References: 1089: Burk KH, et al. Establishment of a human plasma cell line in vitro. Cancer Res. 38: 2508-2513, 1978. PubMed: 566614
1651: Cote RJ, et al. Generation of human monoclonal antibodies reactive with cellular antigens. Proc. Natl. Acad. Sci. USA 80: 2026-2030, 1983. PubMed: 6572959
23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569
26111: Edwards PA, et al. A human-hybridoma system based on a fast-growing mutant of the ARH-77 plasma cell leukemia-derived line. Eur. J. Immunol. 12: 641-648, 1982. PubMed: 7140810
26112: . . Proc. Am. Assoc. Cancer Res. 19: 211, 1978.
43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762
58044: Drewinko B, et al. ARH-77, an established human IgG-producing myeloma cell line. I. Morphology, B-cell phenotypic marker profile, and expression of Epstein-Barr virus. Cancer 54: 1883-1892, 1984. PubMed: 6090003
