ATCC Number：CCL-26?
相關(guān)疾?。赫?br />
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：上皮樣
數(shù)量：大量
器官來(lái)源：腎臟
是否是腫瘤細(xì)胞：0
物種來(lái)源：綠長(zhǎng)尾猴
規(guī)格：0.1ml Designations: BS-C-1
Depositors: ?HE Hopps
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Cercopithecus aethiops
Morphology:epithelial
Source: Organ: kidney
Disease: normal
Cellular Products:keratin
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Applications:transfection host
detection of viruses
Cytogenetic Analysis:Chromosome Frequency Distribution 100 Cells: 2n = 60One large submetacentric marker chromosome. Most of the cells contained a chromosome with a secondary constriction and many of the cells contained dicentric chromosomes.
Comments:The cells are positive for keratin by immunoperoxidase staining.
African green monkey kidney cell line suitable for transfection by SV40 vectors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 1820: Hopps, H. E., et al. Biologic characteristics of the continuous kidney cell line derived from the African green monkey. J. Immunol. 91: 416-424, 1963. PubMed: 14071033
21447: American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.
32372: Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256
32501: Jelachich ML, Lipton HL. Theiler's murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed: 8794327
58406: Schmidt NJ, et al. The sensitivity of grivet monkey kidney cell line BS-C-1 for propagation and isolation of certain human viruses. Am. J. Public Health 54: 1522-1530, 1964.
