-
平臺編號：bio-68285
-
國際編號：CRL-2712?
-
細(xì)胞信息： vT{2}
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費用：
加載中…… - 訂購
-
注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
vT{2}
品系：C57L/J
ATCC Number：CRL-2712?
數(shù)量：大量
器官來源：肝
相關(guān)疾病：肝癌
細(xì)胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
細(xì)胞類型：上皮細(xì)胞
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
規(guī)格：1ml Designations: vT{2}
Depositors: ?O Hankinson
Biosafety Level:2 [Cells contain SV40 and CMV viral DNA ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:epithelial
Source: Organ: liver
Strain: C57L/J
Disease: hepatoma
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:The vT{2} cell line was derived by co-transfection of a 6 thioguanine-resistant derivative of c4 (B13NBii1) [ATCC CRL-2717 ] cell line using the plasmid pSV2gpt and pBM5/NEO-M1-1. M1-1 is a cDNA clone containing the entire human ARNT cDNA sequence. The cells were expanded in G418 to obtain vT{2} (ATCC CRL-2712 ). The vT{2} cell line expresses the human aryl hydrocarbon receptor nuclear translocator (ARNT) gene The vectors contain cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. ARNT is directly involved in the regulation of xenobiotic metabolism (including chemical carcinogenesis), hypoxia and differentiation during embryogeneses. The parental cell line c4 (B13NBii1) (ATCC CRL-2717 ) lacks functional ARNT while its derivative vT{2} (ATCC CRL-2712 ) possesses a complete transfected ARNT cDNA. Together, they can be used to study ARNT processes and the role of ARNT in vivo.
Propagation: ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine supplemented with 0.4 mg/ml G418, 90%; heat-inactivated fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: Every 2 to 3 day
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:parental cell line:ATCC CRL-2717
References: 61153: Hoffman EC, et al. Cloning of a factor required for activity of the Ah (dioxin) receptor. Science 252: 954-958, 1991. PubMed: 1852076
ATCC Number：CRL-2712?
數(shù)量：大量
器官來源：肝
相關(guān)疾病：肝癌
細(xì)胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
細(xì)胞類型：上皮細(xì)胞
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
規(guī)格：1ml Designations: vT{2}
Depositors: ?O Hankinson
Biosafety Level:2 [Cells contain SV40 and CMV viral DNA ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:epithelial
Source: Organ: liver
Strain: C57L/J
Disease: hepatoma
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:The vT{2} cell line was derived by co-transfection of a 6 thioguanine-resistant derivative of c4 (B13NBii1) [ATCC CRL-2717 ] cell line using the plasmid pSV2gpt and pBM5/NEO-M1-1. M1-1 is a cDNA clone containing the entire human ARNT cDNA sequence. The cells were expanded in G418 to obtain vT{2} (ATCC CRL-2712 ). The vT{2} cell line expresses the human aryl hydrocarbon receptor nuclear translocator (ARNT) gene The vectors contain cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. ARNT is directly involved in the regulation of xenobiotic metabolism (including chemical carcinogenesis), hypoxia and differentiation during embryogeneses. The parental cell line c4 (B13NBii1) (ATCC CRL-2717 ) lacks functional ARNT while its derivative vT{2} (ATCC CRL-2712 ) possesses a complete transfected ARNT cDNA. Together, they can be used to study ARNT processes and the role of ARNT in vivo.
Propagation: ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine supplemented with 0.4 mg/ml G418, 90%; heat-inactivated fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: Every 2 to 3 day
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:parental cell line:ATCC CRL-2717
References: 61153: Hoffman EC, et al. Cloning of a factor required for activity of the Ah (dioxin) receptor. Science 252: 954-958, 1991. PubMed: 1852076
