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平臺編號：bio-68295
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國際編號：CCL-149?
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細(xì)胞信息： L2
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
L2
ATCC Number：CCL-149?
數(shù)量：大量
器官來源：肺
細(xì)胞形態(tài)：上皮樣
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：大鼠
生長狀態(tài)：貼壁生長
年限：adult
規(guī)格：0.5mg Designations: L2
Depositors: ?ME Kaighn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Organ: lung
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis:model number = 77; range = 49 to 85
Age: adult
Gender: female
Comments:Gamma glutamylcysteine synthetase activity is stimulated by oxidative stress.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 22220: . . J. Cell Biol. 59: 160a, 1973.
23568: Shi MM, et al. Quinone-induced oxidative stress elevates glutathione and induces gamma- glutamylcysteine synthetase activity in rat lung epithelial L2 cells. J. Biol. Chem. 269: 26512-26517, 1994. PubMed: 7929374
26014: Douglas WH, Kaighn ME. Clonal isolation of differentiated rat lung cells. In Vitro 10: 230-237, 1974. PubMed: 4616002
26015: . . In Vitro 10: 336a, 1974.
26016: . . Pediatr. Res. 9: 276a, 1975.
32967: Su WY, et al. Lung epithelial cell-released nitric oxide protects against PMN-mediated cell injury. Am. J. Physiol. 271: L581-L586, 1996. PubMed: 8897905
33140: Pei L. Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene. J. Biol. Chem. 271: 20879-20884, 1996. PubMed: 8702844
數(shù)量：大量
器官來源：肺
細(xì)胞形態(tài)：上皮樣
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：大鼠
生長狀態(tài)：貼壁生長
年限：adult
規(guī)格：0.5mg Designations: L2
Depositors: ?ME Kaighn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Organ: lung
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis:model number = 77; range = 49 to 85
Age: adult
Gender: female
Comments:Gamma glutamylcysteine synthetase activity is stimulated by oxidative stress.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 22220: . . J. Cell Biol. 59: 160a, 1973.
23568: Shi MM, et al. Quinone-induced oxidative stress elevates glutathione and induces gamma- glutamylcysteine synthetase activity in rat lung epithelial L2 cells. J. Biol. Chem. 269: 26512-26517, 1994. PubMed: 7929374
26014: Douglas WH, Kaighn ME. Clonal isolation of differentiated rat lung cells. In Vitro 10: 230-237, 1974. PubMed: 4616002
26015: . . In Vitro 10: 336a, 1974.
26016: . . Pediatr. Res. 9: 276a, 1975.
32967: Su WY, et al. Lung epithelial cell-released nitric oxide protects against PMN-mediated cell injury. Am. J. Physiol. 271: L581-L586, 1996. PubMed: 8897905
33140: Pei L. Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene. J. Biol. Chem. 271: 20879-20884, 1996. PubMed: 8702844
