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平臺編號：bio-68303
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國際編號：CRL-8146?
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細胞信息： AHH-1
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
AHH-1
生長狀態(tài)：懸浮生長
數(shù)量：大量
細胞形態(tài)：淋巴樣
年限：33 years
細胞類型：B淋巴細胞
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：人
器官來源：外周血
ATCC Number：CRL-8146?
規(guī)格：0.5mg Designations: AHH-1
Depositors: ?Massachusetts Institute of Technology
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10
D5S818: 12,13
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19
Cytogenetic Analysis:near diploid
Age: 33 years
Gender: male
Ethnicity: Caucasian
Comments:The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products. They are are sensitive to killing by benzo[a]pyrene. The cells carry an endogenous Epstein-Barr virus (EBV) genome and are EBNA positive.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; horse serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Growth Conditions: The cells grow as floating aggregates of large cell clusters.
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 16 to 19 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
parental cell line:ATCC CCL-156
derivative:ATCC CRL-10575
References: 21904: Crespi CL, Thilly WG. Mutation assays involving blood cells that metabolize toxic substances. US Patent 4,532,204 dated Jul 30 1985
數(shù)量：大量
細胞形態(tài)：淋巴樣
年限：33 years
細胞類型：B淋巴細胞
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：人
器官來源：外周血
ATCC Number：CRL-8146?
規(guī)格：0.5mg Designations: AHH-1
Depositors: ?Massachusetts Institute of Technology
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10
D5S818: 12,13
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19
Cytogenetic Analysis:near diploid
Age: 33 years
Gender: male
Ethnicity: Caucasian
Comments:The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products. They are are sensitive to killing by benzo[a]pyrene. The cells carry an endogenous Epstein-Barr virus (EBV) genome and are EBNA positive.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; horse serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Growth Conditions: The cells grow as floating aggregates of large cell clusters.
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 16 to 19 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
parental cell line:ATCC CCL-156
derivative:ATCC CRL-10575
References: 21904: Crespi CL, Thilly WG. Mutation assays involving blood cells that metabolize toxic substances. US Patent 4,532,204 dated Jul 30 1985
