![HP [HT1080 poly]](http://www.family998.com/static/images/cells-no_picture.jpg)
-
平臺(tái)編號(hào)：bio-68304
-
國(guó)際編號(hào)：CRL-12012?
-
細(xì)胞信息： HP [HT1080 poly]
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
HP [HT1080 poly]
數(shù)量：大量
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
年限：35 years
ATCC Number：CRL-12012?
相關(guān)疾?。豪w維肉瘤
是否是腫瘤細(xì)胞：0
物種來源：人
規(guī)格：48T Designations: HP [HT1080 poly]
Depositors: ?Chiron Viagene, Inc.
Biosafety Level:2 [Cells contain CMV viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:
Source: Disease: fibrosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19
Age: 35 years
Gender: male
Ethnicity: Caucasian
Comments:The HP polytropic retroviral packaging cell line was derived from the human fibrosarcoma cell line, HT-1080 (see ATCC CCL-121 ).
HT1080 cells were co-transfected with the methotrexate resistance vector, pFR400 and the MoMLV gag/pol expression vector, pSCV10. [57428 ]
After selection for transfected cells, individual drug resistant cell colonies were expanded, analyzed and selected for overexpression of MoMLV gag/pol. [57428 ]
HT1080 (clone SCV21) was co-transfected with the phleomycin resistance vector, pUT507 and the polytropic envelope expression vector, pCMVMCF and subsequently cloned. [57428 ]
The HP clone was selected for expression of relatively high levels of both gag/pol and polytropic envelope proteins. [57428 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 57428: Barber JR, et al. Retroviral packaging cell lines. US Patent 5,591,624 dated Jan 7 1997
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
年限：35 years
ATCC Number：CRL-12012?
相關(guān)疾?。豪w維肉瘤
是否是腫瘤細(xì)胞：0
物種來源：人
規(guī)格：48T Designations: HP [HT1080 poly]
Depositors: ?Chiron Viagene, Inc.
Biosafety Level:2 [Cells contain CMV viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:
Source: Disease: fibrosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19
Age: 35 years
Gender: male
Ethnicity: Caucasian
Comments:The HP polytropic retroviral packaging cell line was derived from the human fibrosarcoma cell line, HT-1080 (see ATCC CCL-121 ).
HT1080 cells were co-transfected with the methotrexate resistance vector, pFR400 and the MoMLV gag/pol expression vector, pSCV10. [57428 ]
After selection for transfected cells, individual drug resistant cell colonies were expanded, analyzed and selected for overexpression of MoMLV gag/pol. [57428 ]
HT1080 (clone SCV21) was co-transfected with the phleomycin resistance vector, pUT507 and the polytropic envelope expression vector, pCMVMCF and subsequently cloned. [57428 ]
The HP clone was selected for expression of relatively high levels of both gag/pol and polytropic envelope proteins. [57428 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
- O.1 mM Non-Essential Amino Acids (NEAA)
- fetal bovine serum to a final concentration of 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
-
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 57428: Barber JR, et al. Retroviral packaging cell lines. US Patent 5,591,624 dated Jan 7 1997
