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平臺編號：bio-68305
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國際編號：CRL-7033?
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細胞信息： Hs 53.T
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Hs 53.T
是否是腫瘤細胞：0
物種來源：人
生長狀態(tài)：貼壁生長
數(shù)量：大量
運輸方式：凍存運輸
年限：13 years adolescent
細胞形態(tài)：成纖維樣
ATCC Number：CRL-7033?
器官來源：皮膚
相關(guān)疾?。浩渌膊?br /> 規(guī)格：0.1ml Designations: Hs 53.T
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: neurofibroma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: January 31, 1969
DNA Profile (STR):Amelogenin: X
CSF1PO: 7,10
D13S317: 9,12
D16S539: 10,12
D5S818: 12,13
D7S820: 12
THO1: 8,9
TPOX: 7,9
vWA: 15,16
Cytogenetic Analysis:modal number = 45; range = 34 to 47
Age: 13 years adolescent
Gender: female
Ethnicity: Black
Comments:Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002
Recommended serum: ATCC 30-2020
0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
Cell culture tested DMSO: ATCC 4-X
物種來源：人
生長狀態(tài)：貼壁生長
數(shù)量：大量
運輸方式：凍存運輸
年限：13 years adolescent
細胞形態(tài)：成纖維樣
ATCC Number：CRL-7033?
器官來源：皮膚
相關(guān)疾?。浩渌膊?br /> 規(guī)格：0.1ml Designations: Hs 53.T
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: neurofibroma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: January 31, 1969
DNA Profile (STR):Amelogenin: X
CSF1PO: 7,10
D13S317: 9,12
D16S539: 10,12
D5S818: 12,13
D7S820: 12
THO1: 8,9
TPOX: 7,9
vWA: 15,16
Cytogenetic Analysis:modal number = 45; range = 34 to 47
Age: 13 years adolescent
Gender: female
Ethnicity: Black
Comments:Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
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Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
- Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
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Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio: 1:2 to 1:3
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002
Recommended serum: ATCC 30-2020
0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
Cell culture tested DMSO: ATCC 4-X
