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數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
ATCC Number：HB-273?
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2a
生長狀態(tài)：懸浮生長
規(guī)格：0.1ml Designations: CC56
Depositors: ?CJ Howard
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against bovine B cell
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with bovine mesenteric lymph node cells.
Spleen cells were fused with NS1 myeloma cells.
The antibody reacts with bovine B cells in peripheral blood and lymph nodes.
It precipitates a 180000 dalton molecule from the surface of bovine B cells.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22775: Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. PubMed: 1708546
24379: . . Vet. Immunol. Immunopathol. 32: 1993..